Constitutive expression of a putative high-affinity nitrate transporter in Nicotiana plumbaginifolia: evidence for post-transcriptional regulation by a reduced nitrogen source
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منابع مشابه
Regulation of high-affinity nitrate transporter genes and high-affinity nitrate influx by nitrogen pools in roots of barley.
To investigate the regulation of HvNRT2, genes that encode high-affinity NO(3)(-) transporters in barley (Hordeum vulgare) roots, seedlings were treated with 10 mM NO(3)(-) in the presence or absence of amino acids (aspartate, asparagine, glutamate [Glu], and glutamine [Gln]), NH(4)(+), and/or inhibitors of N assimilation. Although all amino acids decreased high-affinity (13)NO(3)(-) influx and...
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The high affinity nitrate transport system (HATS) plays an important role in rice nitrogen acquisition because, even under flooded anaerobic cultivation when NH(4)(+) dominates, significant nitrification occurs on the root surface. In the rice genome, four NRT2 and two NAR2 genes encoding HATS components have been identified. One gene OsNRT2.3 was mRNA spliced into OsNRT2.3a and OsNRT2.3b and O...
متن کاملCytokinins and auxins control the expression of a gene in Nicotiana plumbaginifolia cells by feedback regulation.
Both cytokinin (N6-benzyladenine [BA]) and auxin (2,4-dichlorophenoxyacetic acid [2,4-D]) stimulate the accumulation of an mRNA, represented by the cDNA pLS216, in Nicotiana plumbaginifolia suspension culture cells. The kinetics of RNA accumulation were different for the two hormones; however, the response to both was transient, and the magnitude of the response was dose dependent. Runoff trans...
متن کاملTissue-Specific and Pathogen-lnduced Regulation of a Nicotiana plumbaginifolia
The Nicotiana plumbaginifolia gnl gene encoding a 8-1,3-glucanase isoform has been characterired. The gnl product represents an isoform distinct from the previously identified tobacco 8-1,3-glucanases. By expressing gnl in Escherichia coli, we have determined directly that the encoded protein does, indeed, correspond to a &1,3glucanase. In N. plumbaginifolia, gnl was found to be expressed in ro...
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The Stylosanthes hamata SHST1 gene encodes a high-affinity sulfate transporter located in the plasma membrane. In this study the S. hamata SHST1 gene was constitutively expressed in Indian mustard [Brassica juncea (L.) Czern.] to investigate its importance for tolerance and accumulation of various oxyanions that may be transported by SHST1 and for cadmium, which is detoxified by sulfur-rich com...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 2000
ISSN: 0960-7412,1365-313X
DOI: 10.1046/j.1365-313x.2000.00813.x